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spy650 fastact x  (Spirochrome)


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    Spirochrome spy650 fastact x
    Spy650 Fastact X, supplied by Spirochrome, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spy650+fastact+x/SPY650-FastAct_X/bio_rxiv__64898__2026__10__07__757276-201-12-13
    Average 94 stars, based on 6 article reviews
    spy650 fastact x - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Staining:

    Article Title: Machine learning-driven single-cell phenotyping in size-controlled microenvironments via parallel deterministic droplet microfluidics
    Article Snippet: .. Cells were stained membrane-permeable dyes, including calcein AM (cytoplasm; 1 : 1000 dilution; Biotium), Hoechst 33342 (nuclei; 1 : 1000 dilution; Thermo Fisher Scientific), and SPY650-FastAct_X or ‘SiR-XActin’ (F-actin; 1 : 1000 dilution; Spirochrome) for 1 h at 37 °C. .. Full 3D Z -stacks were acquired using a Nikon CSU-W1 SoRa spinning disk confocal microscope.

    Membrane:

    Article Title: Machine learning-driven single-cell phenotyping in size-controlled microenvironments via parallel deterministic droplet microfluidics
    Article Snippet: .. Cells were stained membrane-permeable dyes, including calcein AM (cytoplasm; 1 : 1000 dilution; Biotium), Hoechst 33342 (nuclei; 1 : 1000 dilution; Thermo Fisher Scientific), and SPY650-FastAct_X or ‘SiR-XActin’ (F-actin; 1 : 1000 dilution; Spirochrome) for 1 h at 37 °C. .. Full 3D Z -stacks were acquired using a Nikon CSU-W1 SoRa spinning disk confocal microscope.



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    a , Top-down (top) and oblique angle (middle, bottom) views of still frames from time-lapse 3D SoRa SDCM imaging of LAP2β-AcGFP1 (green) and SPY555_FastAct_X (magenta) in the U2OS cell region confined in a Matrigel/laminin-filled 8 μm-wide pore. Images were rendered and reconstructed in 3D for each time point. Angled side views are more zoomed in on the nuclear envelope, which appears to surround the pore. Nuclear envelope creases and folds (arrows) are seen in the 60 and 120 minute time frames. Note that the nuclear envelope preserves its continuity in the 120 minute timeframe, but the front side becomes dim due to higher signal intensity in the creased regions of the 3D-rendered image. Scale bars, 2 μm. b, Time-lapse frames of a single optical slice from SoRa SDCM imaging of a U2OS cell region confined in a Matrigel/laminin-filled 8 μm-wide pore. U2OS cells were transfected with GFP-SEPT9_i1 (green) and labeled live with <t>SPY650_FastAct_X</t> (magents). White arrows point to actin that colocalizes with GFP-SEPT9_i1, and enlarges overtime. Blue arrows point to actoseptin that appears in frame de novo and remains spatially stable overtime. Scale bars, 2 μm. c, Still frames show side views of 3D volume images from time-lapse 3D SoRa SDCM imaging of GFP-SEPT9_i1 (green) and SPY650_FastAct_X (magenta) in a U2OS cell region confined in a Matrigel/laminin-filled 8 μm-wide pore. Yellow arrowheads point to short-lived actoseptin cables. White arrows point to longer lived actoseptin cables which become brighter and thicker. Blue arrows point to de novo generation of actoseptin cables that grow longitudinally and laterally. Scale bar, 1 μm.
    Spy650 Fastact X, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spy650+fastact+x/SPY650-FastAct/bio_rxiv__64898__2026__01__21__700789-272-29-30
    Average 95 stars, based on 1 article reviews
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    spy650 fastact x  (Spirochrome)
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    a , Top-down (top) and oblique angle (middle, bottom) views of still frames from time-lapse 3D SoRa SDCM imaging of LAP2β-AcGFP1 (green) and SPY555_FastAct_X (magenta) in the U2OS cell region confined in a Matrigel/laminin-filled 8 μm-wide pore. Images were rendered and reconstructed in 3D for each time point. Angled side views are more zoomed in on the nuclear envelope, which appears to surround the pore. Nuclear envelope creases and folds (arrows) are seen in the 60 and 120 minute time frames. Note that the nuclear envelope preserves its continuity in the 120 minute timeframe, but the front side becomes dim due to higher signal intensity in the creased regions of the 3D-rendered image. Scale bars, 2 μm. b, Time-lapse frames of a single optical slice from SoRa SDCM imaging of a U2OS cell region confined in a Matrigel/laminin-filled 8 μm-wide pore. U2OS cells were transfected with GFP-SEPT9_i1 (green) and labeled live with <t>SPY650_FastAct_X</t> (magents). White arrows point to actin that colocalizes with GFP-SEPT9_i1, and enlarges overtime. Blue arrows point to actoseptin that appears in frame de novo and remains spatially stable overtime. Scale bars, 2 μm. c, Still frames show side views of 3D volume images from time-lapse 3D SoRa SDCM imaging of GFP-SEPT9_i1 (green) and SPY650_FastAct_X (magenta) in a U2OS cell region confined in a Matrigel/laminin-filled 8 μm-wide pore. Yellow arrowheads point to short-lived actoseptin cables. White arrows point to longer lived actoseptin cables which become brighter and thicker. Blue arrows point to de novo generation of actoseptin cables that grow longitudinally and laterally. Scale bar, 1 μm.
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    a , Top-down (top) and oblique angle (middle, bottom) views of still frames from time-lapse 3D SoRa SDCM imaging of LAP2β-AcGFP1 (green) and SPY555_FastAct_X (magenta) in the U2OS cell region confined in a Matrigel/laminin-filled 8 μm-wide pore. Images were rendered and reconstructed in 3D for each time point. Angled side views are more zoomed in on the nuclear envelope, which appears to surround the pore. Nuclear envelope creases and folds (arrows) are seen in the 60 and 120 minute time frames. Note that the nuclear envelope preserves its continuity in the 120 minute timeframe, but the front side becomes dim due to higher signal intensity in the creased regions of the 3D-rendered image. Scale bars, 2 μm. b, Time-lapse frames of a single optical slice from SoRa SDCM imaging of a U2OS cell region confined in a Matrigel/laminin-filled 8 μm-wide pore. U2OS cells were transfected with GFP-SEPT9_i1 (green) and labeled live with <t>SPY650_FastAct_X</t> (magents). White arrows point to actin that colocalizes with GFP-SEPT9_i1, and enlarges overtime. Blue arrows point to actoseptin that appears in frame de novo and remains spatially stable overtime. Scale bars, 2 μm. c, Still frames show side views of 3D volume images from time-lapse 3D SoRa SDCM imaging of GFP-SEPT9_i1 (green) and SPY650_FastAct_X (magenta) in a U2OS cell region confined in a Matrigel/laminin-filled 8 μm-wide pore. Yellow arrowheads point to short-lived actoseptin cables. White arrows point to longer lived actoseptin cables which become brighter and thicker. Blue arrows point to de novo generation of actoseptin cables that grow longitudinally and laterally. Scale bar, 1 μm.
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    Image Search Results


    a , Top-down (top) and oblique angle (middle, bottom) views of still frames from time-lapse 3D SoRa SDCM imaging of LAP2β-AcGFP1 (green) and SPY555_FastAct_X (magenta) in the U2OS cell region confined in a Matrigel/laminin-filled 8 μm-wide pore. Images were rendered and reconstructed in 3D for each time point. Angled side views are more zoomed in on the nuclear envelope, which appears to surround the pore. Nuclear envelope creases and folds (arrows) are seen in the 60 and 120 minute time frames. Note that the nuclear envelope preserves its continuity in the 120 minute timeframe, but the front side becomes dim due to higher signal intensity in the creased regions of the 3D-rendered image. Scale bars, 2 μm. b, Time-lapse frames of a single optical slice from SoRa SDCM imaging of a U2OS cell region confined in a Matrigel/laminin-filled 8 μm-wide pore. U2OS cells were transfected with GFP-SEPT9_i1 (green) and labeled live with SPY650_FastAct_X (magents). White arrows point to actin that colocalizes with GFP-SEPT9_i1, and enlarges overtime. Blue arrows point to actoseptin that appears in frame de novo and remains spatially stable overtime. Scale bars, 2 μm. c, Still frames show side views of 3D volume images from time-lapse 3D SoRa SDCM imaging of GFP-SEPT9_i1 (green) and SPY650_FastAct_X (magenta) in a U2OS cell region confined in a Matrigel/laminin-filled 8 μm-wide pore. Yellow arrowheads point to short-lived actoseptin cables. White arrows point to longer lived actoseptin cables which become brighter and thicker. Blue arrows point to de novo generation of actoseptin cables that grow longitudinally and laterally. Scale bar, 1 μm.

    Journal: bioRxiv

    Article Title: Septins buffer actomyosin forces to protect the nucleus from genotoxic mechanical stress

    doi: 10.64898/2026.01.21.700789

    Figure Lengend Snippet: a , Top-down (top) and oblique angle (middle, bottom) views of still frames from time-lapse 3D SoRa SDCM imaging of LAP2β-AcGFP1 (green) and SPY555_FastAct_X (magenta) in the U2OS cell region confined in a Matrigel/laminin-filled 8 μm-wide pore. Images were rendered and reconstructed in 3D for each time point. Angled side views are more zoomed in on the nuclear envelope, which appears to surround the pore. Nuclear envelope creases and folds (arrows) are seen in the 60 and 120 minute time frames. Note that the nuclear envelope preserves its continuity in the 120 minute timeframe, but the front side becomes dim due to higher signal intensity in the creased regions of the 3D-rendered image. Scale bars, 2 μm. b, Time-lapse frames of a single optical slice from SoRa SDCM imaging of a U2OS cell region confined in a Matrigel/laminin-filled 8 μm-wide pore. U2OS cells were transfected with GFP-SEPT9_i1 (green) and labeled live with SPY650_FastAct_X (magents). White arrows point to actin that colocalizes with GFP-SEPT9_i1, and enlarges overtime. Blue arrows point to actoseptin that appears in frame de novo and remains spatially stable overtime. Scale bars, 2 μm. c, Still frames show side views of 3D volume images from time-lapse 3D SoRa SDCM imaging of GFP-SEPT9_i1 (green) and SPY650_FastAct_X (magenta) in a U2OS cell region confined in a Matrigel/laminin-filled 8 μm-wide pore. Yellow arrowheads point to short-lived actoseptin cables. White arrows point to longer lived actoseptin cables which become brighter and thicker. Blue arrows point to de novo generation of actoseptin cables that grow longitudinally and laterally. Scale bar, 1 μm.

    Article Snippet: Actin was labeled in fixed cells with AF488 Phalloidin (AAT Bioquest, 23153) and Phalloidin-iFluor 647 (Abcam, ab176759), and in live cells with SPY555-Actin (Cytoskeleton, CY-SC202), SPY555-FastAct (Cytoskeleton, CY-SC205) or SPY650-FastAct_X (Cytoskeleton, CY-SC502).

    Techniques: Imaging, Transfection, Labeling